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[Beckman Coulter] - CytoFLEX 유세포 분석 구성
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2024-04-08
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Flow-cytometry-brochure-cytoflex-nano.pdf (5.4 MB)
The CytoFLEX nano Flow Cytometer is the first nanoscale flow cytometer
Launch of the CytoFLEX nano Flow Cytometer
Explore the full picture of your EV experiment with the CytoFLEX nano flow cytometer.
With the ability to count, size, and characterize EVs using a single technique, it propels your research forward.
Experience greater sensitivity, consistent instrument performance, and flexibility to study your sample.
With the CytoFLEX nano flow cytometer, we have lowered the limits of detection, so you can achieve more.
With the ability to count, size, and characterize EVs using a single technique, it propels your research forward.
Experience greater sensitivity, consistent instrument performance, and flexibility to study your sample.
With the CytoFLEX nano flow cytometer, we have lowered the limits of detection, so you can achieve more.
Research use only. Not for use in diagnostic procedures.
Meet the CytoFLEX nano Flow Cytometer. A pivotal development in flow cytometry, the CytoFLEX nano analyzer enables the analysis of EVs at least as small as 40 nm* with ease while simultaneously offering up to 6 separate fluorescent channels of detection, to deliver full characterization.
With the CytoFLEX nano Flow Cytometer, you can expect:
- Increased size sensitivity from 1 μm to at least 40 nm*
- Flexibility for your experimental design with 6 fluorescent channels and 5 side scatter channels
- Consistency of results with >90% volumetric counting accuracy and <1% carryover between samples
*Characterized using 40 nm polystyrene beads and triggering on violet side scatter.
PERFORMANCE
| CytoFLEX nano Flow Cytometer Performance | ||
| Violet side scatter sensitivity | VSSC1: 40 nm relative to polystyrene nanoparticles VSSC2: 80 nm relative to polystyrene nanoparticles | |
| Scatter detection dynamic range | VSSC 1 for small range 40-150 nm polystyrene nanoparticles VSSC2 for large range 80-1000 nm polystyrene nanoparticles | |
| Violet forward scatter sensitivity | 300 nm relative to polystyrene nanoparticles | |
| Fluorescence sensitivity and resolution | Simultaneous detection of fluorescence on six fluorescence detectors, using 500 nm CytoFLEX nano Multi-intensity Fluorospheres V477: 8 peaks B531: 8 peaks Y595: 8 peaks R670: 6 peaks R710: 5 peaks R792: 4 peaks | |
| Fluorescence rCV | CV < 10% (using QC Fluorospheres at 1 µL/min) | |
| Carryover between Samples | ≤1%a | |
| Acquisition speed | Maximum electronic acquisition speed | 16000 events/second with ≤ 95% yield |
| Recommended maximum sample acquisition speed | 5000 events/second to avoid possible swarming or coincidence situations. | |
| Volumetric Counting accuracy | > 90%b | |
a. Tested with polystyrene nanoparticles.
b. Tested with 144 nm QC Scatterspheres on 3 units under the conditions: record 3 minutes at the sample flow rates of 1μL/min, 2μL/min, 3μL/min,
4μL/min, 5μL/min, 6μL/min respectively, repeat 5 times, then calculated the average total events for each speed, compared the calculated the
volumetric counting accuracy with the theoretical total events (the known concentration 1.3*108 x volume).
For more information:
- 전화번호
- 02-2138-7811
- 이메일
- duuree@dreamcell.co.kr