| Description | This Mouse TNF ELISA Kit was designed for the quantitative measurement of Mouse TNF protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL. |
| Alternative Names | Tnf ELISA Kit; Tnfa ELISA Kit; Tnfsf2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit |
| Abbreviation | TNF |
| Uniprot No. | P06804 |
| Species | Mus musculus (Mouse) |
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates. |
| Detection Range | 15.6 pg/ml-1000 pg/ml. |
| Sensitivity | 3.9 pg/ml |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology
|
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Materials provided | - An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
- Two bottles of lyophilized standard --- Reconstitute one bottle of standard with sample diluent at dilution series and draw the standard curve.
- Biotin-labeled TNF-α antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
- HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
- Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
- HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
- Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
- Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound solution and non-specific substances.
- TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Seal the microtiter plate when incubation.
- An instruction manual
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
