(주) 드림셀-생명공학 실험실용 시약, 기기, 소모품

홈 > 제품정보 > 시약 > 해외 공식 대리점 > [io1098] CRISPRi-Ready ioGlutamatergic Neurons

제품코드 : io1098

제품정보 : CRISPRi-Ready ioGlutamatergic Neurons

Human iPSC-derived glutamatergic neurons ready for gene repressions and CRISPRi screens CRISPR interference (CRISPRi)-Ready ioGlutamatergic Neurons are opti‑ox™ deterministically programmed excitatory neurons that stably express catalytically inactive Cas9 nuclease (dCas9) fused to a transcriptional repression domain. The cells arrive ready for guide RNA (gRNA) delivery from day 1 post-thaw, and high levels of dCas9 expression are maintained for at least 21 days. Using our optimised lentivirus gRNA delivery protocol, users can perform gene repressions, pooled or arrayed CRISPR repression screens and start measuring readouts within a few days. Using CRISPRi-Ready, CRISPRa-Ready and CRISPRko-Ready ioGlutamatergic Neurons eliminates the need to spend months engineering and characterising Cas9-stable iPSC lines and optimising differentiation protocols, significantly reducing experimental timelines. With these ready-to-use cells, reliable and reproducible experimental results can be achieved by simply introducing gRNAs targeting the gene of interest. The cells are a powerful tool for functional genomics, drug target identification and translational research. Applications: Gene repressions, Pooled CRISPR interference screens, Arrayed CRISPR interference screens

인류의 건강과 생명공학 발전을 위해 봉사하는 기업 드림셀