(주) 드림셀-생명공학 실험실용 시약, 기기, 소모품

홈 > 제품정보 > 시약 > 해외 공식 대리점 > [io1099] CRISPRa-Ready ioGlutamatergic Neurons

제품코드 : io1099

제품정보 : CRISPRa-Ready ioGlutamatergic Neurons

Human iPSC-derived glutamatergic neurons ready for gene activations and CRISPR screens CRISPR activation (CRISPRa)-Ready ioGlutamatergic Neurons are opti‑ox deterministically programmed excitatory neurons that constitutively express catalytically inactive Cas9 nuclease (dCas9) fused to a transcriptional activation domain. The cells arrive ready for guide RNA (gRNA) delivery from day 1 post-thaw, and high levels of dCas9 expression are maintained for at least 21 days. Using our optimised lentivirus gRNA delivery protocol, users can perform gene activations, pooled or arrayed CRISPR activation screens and start measuring readouts within a few days. Using CRISPRa-Ready, CRISPRi-Ready and CRISPRko-Ready ioGlutamatergic Neurons eliminates the need to spend months engineering and characterising Cas9-stable iPSC lines and optimising differentiation protocols, significantly reducing experimental timelines. With these ready-to-use cells, reliable and reproducible experimental results can be achieved by simply introducing gRNAs targeting the gene of interest. The cells are a powerful tool for functional genomics, drug target identification and translational research. Applications: Gene activations, Pooled CRISPR activation screens, #Arrayed CRISPR activation screens

인류의 건강과 생명공학 발전을 위해 봉사하는 기업 드림셀