| Description | The Mouse luteinizing hormone (LH) ELISA kit is designed for detection and quantitation of human IL-1β in serum, plasma, or tissue homogenates. It has been validated with high sensitivity, strong specificity, good linearity, precision less than 15%, and consistency across batch. Refer to the product instructions for more details. The measurement of this assay is based on the Sandwich-ELISA tecnique. The samples and biotin-labeled LH antibody are successively pipetted into the wells pre-coated with anti-mouse LH antibody. HRP-avidin is subsequently added to the wells. After thorough washing, th...Read more |
| Alternative Names | LhbLutropin subunit beta ELISA kit; Lutropin beta chain ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit |
| Abbreviation | LH |
| Uniprot No. | O09108 |
| Species | Mus musculus (Mouse) |
| Sample Types | |
| Detection Range | |
| Sensitivity | |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Signal Transduction
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| Assay Principle | quantitative |
| Measurement | Sandwich |
| Materials provided | - A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse LH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Six vials lyophilized standard (0.5 ml/bottle)--- Dilute a bottle ofthe standard at dilution series, read the OD values, and then draw a standard curve.
- Biotin-labeled LH antibody 1 x 6 ml---Act as the detection antibody.
- HRP-avidin 1 x 11ml --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- Wash Buffer (20 x concentrate) 1 x 15 ml --- Wash away unbound or free substances.
- Substrate A 1 x 7 ml --- Chromogenic agent A acts as a hydrogen donor.
- Substrate B 1 x 7 ml --- Chromogenic agent B (including TMB) is used for color developing.
- Stop Solution 1 x 7 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
