| Description | This Mouse IL1A ELISA Kit was designed for the quantitative measurement of Mouse IL1A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 2.35 pg/ml - 150 pg/ml and the sensitivity is 0.58 pg/ml. |
| Target Name | interleukin 1, alpha |
| Alternative Names | Il1aInterleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit |
| Abbreviation | IL1A |
| Uniprot No. | P01582 |
| Species | Mus musculus (Mouse) |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 2.35 pg/ml - 150 pg/ml |
| Sensitivity | 0.58 pg/ml |
| Assay Time | 1-5h |
| Sample Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology
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| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | |
| Linearity | |
| Recovery | |
| Typical Data |
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| Materials provided | - A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse IL-1α antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled IL-1α antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs | ELISA kit FAQs |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Lead Time | 3-5 working days |
