| Description | This Mouse IL1B ELISA Kit was designed for the quantitative measurement of Mouse IL1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.  | 
| Alternative Names | Il1bInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit | 
| Abbreviation | IL1B | 
| Uniprot No. | P10749 | 
| Species | Mus musculus (Mouse) | 
| Sample Types | serum, plasma, cell culture supernates, tissue homogenates. | 
| Detection Range | 31.25 pg/ml-2000 pg/ml. | 
| Sensitivity | 7.8 pg/ml. | 
| Assay Time | 1-5h | 
| Sample Volume | 50-100ul | 
| Detection Wavelength | 450 nm | 
| Research Area | Immunology
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| Assay Principle | quantitative | 
| Measurement | Sandwich | 
| Materials provided | - A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse IL-1β This dismountable microplate can be divided into 12 x 8 strip plates.
 - Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
 - Biotin-labeled IL-1β antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
 - HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
 - Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
 - HRP-avidin Diluent 1 x 15 ml ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
 - Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
 - Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
 - TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
 - Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
 - Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
 - An instruction manual
 
 
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
 - An incubator can provide stable incubation conditions up to 37°C±5°C.
 - Centrifuge
 - Vortex
 - Squirt bottle, manifold dispenser, or automated microplate washer
 - Absorbent paper for blotting the microtiter plate
 - 50-300ul multi-channel micropipette
 - Pipette tips
 - Single-channel micropipette with different ranges
 - 100ml and 500ml graduated cylinders
 - Deionized or distilled water
 - Timer
 - Test tubes for dilution
 
 
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed | 
Troubleshooting and FAQs | ELISA kit FAQs | 
| Storage | Store at 2-8°C. Please refer to protocol. | 
| Lead Time | 3-5 working days |