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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-ET011614MO] Mouse pro interleukin 1 beta (pro-IL1B) ELISA Kit
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[CSB-ET011614MO] Mouse pro interleukin 1 beta (pro-IL1B) ELISA Kit

제품코드 : CSB-ET011614MO

제품정보 : Mouse pro interleukin 1 beta (pro-IL1B) ELISA Kit

  • Sample Types: serum, plasma, cell culture supernates, tissue homogenates
  • Detect Range: 6.25 pg/mL-400 pg/mL
  • Sensitivity: 1.56 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Mouse pro-IL1B ELISA Kit was designed for the quantitative measurement of Mouse pro-IL1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

Target Namepro interleukin 1 beta (pro-IL1B)
Abbreviationpro-IL1B
SpeciesMus musculus (Mouse)
Sample Typesserum, plasma, cell culture supernates, tissue homogenates
Detection Range6.25 pg/mL-400 pg/mL
Sensitivity1.56 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse pro-IL1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %106
Range %101-113
1:2Average %87
Range %82-91
1:4Average %94
Range %90-99
1:8Average %85
Range %80-90
Recovery
The recovery of mouse pro-IL1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9691-100
EDTA plasma (n=4)9185-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
4002.4962.3622.4292.282
2001.9511.8411.8961.749
1001.3491.3191.3341.187
500.9350.8850.9100.763
250.6030.6270.6150.468
12.50.3670.3740.3710.224
6.250.2280.2360.2320.085
00.1440.1500.147　
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations