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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E07435r] Rat Interleukin 5,IL-5 ELISA KIT
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[CSB-E07435r] Rat Interleukin 5,IL-5 ELISA KIT

제품코드 : CSB-E07435r

제품정보 : Rat Interleukin 5,IL-5

  • Sample Types: serum, plasma, cell culture supernates, tissue homogenates
  • Detect Range: 15.6 pg/mL-1000 pg/mL
  • Sensitivity: 3.9 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Rat IL5 ELISA Kit was designed for the quantitative measurement of Rat IL5 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

Target Nameinterleukin 5 (colony-stimulating factor, eosinophil)
Alternative NamesIl5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
AbbreviationIL5
Uniprot No.Q08125
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, cell culture supernates, tissue homogenates
Detection Range15.6 pg/mL-1000 pg/mL
Sensitivity3.9 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%   
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10%   
Three samples of known concentration were tested in twenty assays to assess.  
       
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4) 
1:1Average %85 
Range %82-91 
1:2Average %92 
Range %83-99 
1:4Average %101 
Range %93-107 
1:8Average %96 
Range %90-104 
Recovery
The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange 
Serum (n=5)8885-94 
EDTA plasma (n=4)9490-100 
       
       
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected 
10002.4072.4572.4322.268 
5001.7681.7361.7521.588 
2501.0651.0981.0820.918 
1250.7040.7350.7200.556 
62.50.4480.4690.4590.295 
31.20.3230.3000.3120.148 
15.60.2210.2290.2250.061 
00.1610.1670.164　 
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations