| Description | This Human IL-12/P70 ELISA Kit was designed for the quantitative measurement of Human IL-12/P70 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/ml-2000 pg/ml and the sensitivity is 7.81 pg/ml.  | 
| Abbreviation | IL-12/P70 | 
| Species | Homo sapiens (Human) | 
| Sample Types | serum, plasma, cell culture supernates | 
| Detection Range | 31.25 pg/ml - 2000pg/ml | 
| Sensitivity | 7.8 pg/ml | 
| Assay Time | 1-5h | 
| Sample Volume | 50-100ul | 
| Detection Wavelength | 450 nm | 
| Research Area | Immunology
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| Assay Principle | quantitative | 
| Measurement | Sandwich | 
| Materials provided | - A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human IL-12 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
 - Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
 - One vial Biotin-labeled IL-12 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
 - One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
 - One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
 - One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
 - One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
 - One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
 - One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
 - One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
 - Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
 - An instruction manual
 
 
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| Materials not provided | - A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
 - An incubator can provide stable incubation conditions up to 37°C±5°C.
 - Centrifuge
 - Vortex
 - Squirt bottle, manifold dispenser, or automated microplate washer
 - Absorbent paper for blotting the microtiter plate
 - 50-300ul multi-channel micropipette
 - Pipette tips
 - Single-channel micropipette with different ranges
 - 100ml and 500ml graduated cylinders
 - Deionized or distilled water
 - Timer
 - Test tubes for dilution
 
 
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| ELISA Data Analysis | Watch ELISA data processing video & download Curve Expert if needed | 
Troubleshooting and FAQs | ELISA kit FAQs | 
| Storage | Store at 2-8°C. Please refer to protocol. | 
| Lead Time | 3-5 working days |