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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E07362r] Rat Interleukin 12,IL-12/P40 ELISA KIT
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[CSB-E07362r] Rat Interleukin 12,IL-12/P40 ELISA KIT

제품코드 : CSB-E07362r

제품정보 : Rat Interleukin 12,IL-12/P40

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 31.25 pg/mL-2000 pg/mL
  • Sensitivity: 7.81 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description
The rat NF-κB subunit p65 ELISA kit is suitable for the quantitative determination of rat p65 in different sample types, including serum, plasma, and tissue homogenates. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products is positively related to the analyte of interest in the sample. IL-12 is a Th1 cytokine predominantly produced by cells in the innate immune system. Its role is linked to the differentiation of naïve T cells into Th1 cells. In allergy, IL-12 is know...Read more
Target NameInterleukin 12,IL-12/P40
Alternative NamesN/A
AbbreviationIL12/P40
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, tissue homogenates
Detection Range31.25 pg/mL-2000 pg/mL
Sensitivity7.81 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %102
Range %96-106
1:2Average %94
Range %90-98
1:4Average %95
Range %90-99
1:8Average %89
Range %82-94
Recovery
The recovery of rat IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)9892-104
EDTA plasma (n=4)10397-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.3292.2282.2792.077
10001.7481.6471.6981.496
5001.1521.1311.1420.940
2500.7940.7830.7890.587
1250.5060.4950.5010.299
62.50.4150.4040.4100.208
31.250.3080.3030.3060.104
00.2020.2010.202　
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations