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Product Details

[CSB-E07364r] Rat Interleukin 12,IL-12/P70 ELISA KIT

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E07364r] Rat Interleukin 12,IL-12/P70 ELISA KIT

제품코드 : CSB-E07364r

제품정보 : Rat Interleukin 12,IL-12/P70

Sample Types: serum, plasma, tissue homogenates
Detect Range: 3.12 pg/mL-200 pg/mL
Sensitivity: 0.78 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Rat IL12/P70 ELISA Kit was designed for the quantitative measurement of Rat IL12/P70 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

Target Name

Interleukin 12,IL-12/P70

Alternative Names

N/A

Abbreviation

IL12/P70

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-106
1:2	Average %	94
	Range %	91-98
1:4	Average %	93
	Range %	89-96
1:8	Average %	87
	Range %	82-96

Recovery

The recovery of rat IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	103	98-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.557	2.656	2.607	2.425
100	2.023	2.122	2.073	1.891
50	1.448	1.468	1.458	1.276
25	0.928	0.917	0.923	0.741
12.5	0.578	0.569	0.574	0.392
6.25	0.415	0.405	0.410	0.228
3.12	0.303	0.308	0.306	0.124
0	0.181	0.182	0.182