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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E07452r] Rat Interleukin 16,IL-16 ELISA KIT
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[CSB-E07452r] Rat Interleukin 16,IL-16 ELISA KIT

제품코드 : CSB-E07452r

제품정보 : Rat Interleukin 16,IL-16

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 31.25 pg/mL-2000 pg/mL
  • Sensitivity: 7.81 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Rat IL16 ELISA Kit was designed for the quantitative measurement of Rat IL16 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Target NameInterleukin 16,IL-16
AbbreviationIL16
Uniprot No.G5BEZ3
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, tissue homogenates
Detection Range31.25 pg/mL-2000 pg/mL
Sensitivity7.81 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%   
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10%   
Three samples of known concentration were tested in twenty assays to assess.  
       
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4) 
1:1Average %92 
Range %87-96 
1:2Average %98 
Range %94-102 
1:4Average %103 
Range %98-107 
1:8Average %89 
Range %84-96 
Recovery
The recovery of rat IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange 
Serum (n=5)9490-98 
EDTA plasma (n=4)8882-96 
       
       
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected 
20002.7572.6562.7072.524 
10002.2232.1222.1731.990 
5001.4881.4671.4781.295 
2500.9370.9260.9320.749 
1250.5890.5780.5840.401 
62.50.4250.4140.4200.237 
31.250.3050.2970.3010.118 
00.1830.1820.183　 
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations