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Product Details

[CSB-E11707h] Human Interleukin 21,IL-21 ELISA Kit

제품문의

제품상세

홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E11707h] Human Interleukin 21,IL-21 ELISA Kit

제품코드 : CSB-E11707h

제품정보 : Human Interleukin 21,IL-21

Sample Types: serum, plasma, tissue homogenates
Detect Range: 3.12 pg/mL-200 pg/mL
Sensitivity: 0.78 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Human IL21 ELISA Kit was designed for the quantitative measurement of Human IL21 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

Target Name

interleukin 21

Alternative Names

CVID11 ELISA Kit; IL 21 ELISA Kit; IL-21 ELISA Kit; Il21 ELISA Kit; IL21_HUMAN ELISA Kit; Interleukin 21 ELISA Kit; Interleukin-21 ELISA Kit; interleukin-21 isoform ELISA Kit; Interleukin21 ELISA Kit; OTTHUMP00000164088 ELISA Kit; Za11 ELISA Kit

Abbreviation

IL21

Uniprot No.

Q9HBE4

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

3.12 pg/mL-200 pg/mL

Sensitivity

0.78 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-96
1:2	Average %	92
	Range %	87-99
1:4	Average %	87
	Range %	84-92
1:8	Average %	94
	Range %	87-100

Recovery

The recovery of human IL-21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	101	85-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.445	2.345	2.395	2.250
100	1.864	1.764	1.814	1.669
50	1.162	1.151	1.157	1.012
25	0.723	0.713	0.718	0.573
12.5	0.432	0.422	0.427	0.282
6.25	0.315	0.305	0.310	0.165
3.12	0.222	0.212	0.217	0.072
0	0.145	0.144	0.145