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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E07379r] Rat Tumor necrosis factor β,TNF-β ELISA KIT
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[CSB-E07379r] Rat Tumor necrosis factor β,TNF-β ELISA KIT

제품코드 : CSB-E07379r

제품정보 : Rat Tumor necrosis factor β,TNF-β

  • Sample Types: serum, plasma, tissue homogenates
  • Detect Range: 7.8 pg/mL-500 pg/mL
  • Sensitivity: 1.95 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Rat TNF-β ELISA Kit was designed for the quantitative measurement of Rat TNF-β protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Target NameTumor necrosis factor β,TNF-β
Alternative NamesLta ELISA Kit; Tnfb ELISA Kit; Tnfsf1 ELISA Kit; Lymphotoxin-alpha ELISA Kit; LT-alpha ELISA Kit; TNF-beta ELISA Kit; Tumor necrosis factor ligand superfamily member 1 ELISA Kit
AbbreviationTNF-β
Uniprot No.Q06332
SpeciesRattus norvegicus (Rat)
Sample Typesserum, plasma, tissue homogenates
Detection Range7.8 pg/mL-500 pg/mL
Sensitivity1.95 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TNF-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　SampleSerum(n=4)
1:1Average %101
Range %96-105
1:2Average %102
Range %97-107
1:4Average %87
Range %83-94
1:8Average %96
Range %91-102
Recovery
The recovery of rat TNF-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5)10499108
EDTA plasma (n=4)8884-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.6322.7652.6992.614
2502.2122.3022.2572.172
1251.5451.5771.5611.476
62.50.7650.7710.7680.683
31.20.4110.4230.4170.332
15.60.2570.2640.2610.176
7.80.1760.1850.1810.096
00.0840.0850.085　
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

Citations