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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL004895RA] Rat OX-2 membrane glycoprotein(CD200) ELISA kit

[CSB-EL004895RA] Rat OX-2 membrane glycoprotein(CD200) ELISA kit

제품코드 : CSB-EL004895RA

제품정보 : Rat OX-2 membrane glycoprotein(CD200)

Sample Types: serum, plasma, tissue homogenates
Detect Range: 47 pg/mL-3000 pg/mL
Sensitivity: 11.75 pg/mL

제품문의

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

제품상세

Product Details

Description

This Rat CD200 ELISA Kit was designed for the quantitative measurement of Rat CD200 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.

Target Name

CD200 molecule

Alternative Names

Cd200; Mox2; OX-2 membrane glycoprotein; MRC OX-2 antigen; CD antigen CD200

Abbreviation

CD200

Uniprot No.

P04218

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

47 pg/mL-3000 pg/mL

Sensitivity

11.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CD200 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	90
	Range %	86-94
1:4	Average %	95
	Range %	90-100
1:8	Average %	92
	Range %	88-96

Recovery

The recovery of rat CD200 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	106	102-113
EDTA plasma (n=4)	98	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.271	2.317	2.294	2.103
1500	1.429	1.369	1.399	1.208
750	0.928	0.984	0.956	0.765
375	0.589	0.568	0.579	0.388
187.5	0.396	0.372	0.384	0.193
94	0.288	0.304	0.296	0.105
47	0.240	0.231	0.236	0.045
0	0.188	0.193	0.191