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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL004940HU] Human Leukocyte surface antigen CD47(CD47) ELISA kit
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[CSB-EL004940HU] Human Leukocyte surface antigen CD47(CD47) ELISA kit

제품코드 : CSB-EL004940HU

제품정보 : Human Leukocyte surface antigen CD47(CD47)

  • Sample Types: serum, plasma, urine, tissue homogenates
  • Detect Range: 3.12 pg/mL-200 pg/mL
  • Sensitivity: 0.78 pg/mL

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  • 전화번호
    2292-8870

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Product Details

Description

This Human CD47 ELISA Kit was designed for the quantitative measurement of Human CD47 protein in serum, plasma, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

Target NameCD47 molecule
Alternative NamesAntigen identified by monoclonal ELISA Kit 1D8 ELISA Kit; Antigenic surface determinant protein OA3 ELISA Kit; CD 47 ELISA Kit; CD47 ELISA Kit; CD47 antigen (Rh-related antigen; integrin-associated signal transducer) ELISA Kit; CD47 antigen ELISA Kit; CD47 glycoprotein ELISA Kit; CD47 molecule ELISA Kit; CD47_HUMAN ELISA Kit; IAP ELISA Kit; Integrin Associated Protein ELISA Kit; Integrin associated signal transducer ELISA Kit; Integrin-associated protein ELISA Kit; Leukocyte surface antigen CD47 ELISA Kit; MER 6 ELISA Kit; MER6 ELISA Kit; OA 3 ELISA Kit; OA3 ELISA Kit; OTTHUMP00000041152 ELISA Kit; OTTHUMP00000041153 ELISA Kit; Protein MER6 ELISA Kit; Rh related antigen ELISA Kit; Surface antigen identified by monoclonal ELISA Kit 1D8 ELISA Kit
AbbreviationCD47
Uniprot No.Q08722
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma, urine, tissue homogenates
Detection Range3.12 pg/mL-200 pg/mL
Sensitivity0.78 pg/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%    
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%    
Three samples of known concentration were tested in twenty assays to assess.   
        
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD47 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. 
　SampleSerum(n=4)  
1:1Average %96  
Range %90-101  
1:2Average %106  
Range %102-109  
1:4Average %94  
Range %90-98  
1:8Average %88  
Range %85-94  
Recovery
The recovery of human CD47 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. 
 
Sample TypeAverage % RecoveryRange  
Serum (n=5)10399-106  
EDTA plasma (n=4)9391-95  
        
        
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
 
pg/mlOD1OD2AverageCorrected  
2001.9411.9251.9331.802  
1001.6401.5191.5801.449  
501.1221.1321.1270.996  
250.6740.6240.6490.518  
12.50.4410.4500.4460.315  
6.250.3010.3270.3140.183  
3.120.2030.1940.1990.068  
00.1330.1280.131　  
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

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