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Product Details

[CSB-E15792h] Human soluble Cluster of differentiation 100(sCD100)ELISA kit

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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E15792h] Human soluble Cluster of differentiation 100(sCD100)ELISA kit

제품코드 : CSB-E15792h

제품정보 : Human soluble Cluster of differentiation 100(sCD100

Sample Types: serum, plasma, tissue homogenates
Detect Range: 125 pg/mL-8000 pg/mL
Sensitivity: 31.25 pg/mL

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Description

This Human SEMA4D ELISA Kit was designed for the quantitative measurement of Human SEMA4D protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL .

Target Name

sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D

Alternative Names

A8 ELISA Kit; BB 18 ELISA Kit; BB18 ELISA Kit; CD 100 ELISA Kit; CD100 ELISA Kit; CD100 antigen ELISA Kit; Coll 4 ELISA Kit; COLL4 ELISA Kit; Collapsin 4 ELISA Kit; Collapsin4 ELISA Kit; GR3 ELISA Kit; Leukocyte activation antigen CD100 ELISA Kit; M sema G ELISA Kit; MSEMA ELISA Kit; SEM4D_HUMAN ELISA Kit; Sema 4d ELISA Kit; Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D ELISA Kit; Sema H ELISA Kit; SEMA J ELISA Kit; Sema4d ELISA Kit; Semacl 2 ELISA Kit; Semacl2 ELISA Kit; SemaH ELISA Kit; SEMAJ ELISA Kit; Semaphorin 4D ELISA Kit; Semaphorin C like 2 ELISA Kit; Semaphorin H ELISA Kit; Semaphorin J ELISA Kit; Semaphorin-4D ELISA Kit; Semaphorin4D ELISA Kit; SemaphorinJ ELISA Kit; Semcl 2 ELISA Kit; Semcl2 ELISA Kit

Abbreviation

SEMA4D

Uniprot No.

Q92854

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

125 pg/mL-8000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human sCD100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-102
1:2	Average %	93
	Range %	87-100
1:4	Average %	94
	Range %	88-102
1:8	Average %	92
	Range %	84-97

Recovery

The recovery of human sCD100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-106
EDTA plasma (n=4)	92	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	1.850	1.835	1.843	1.755
4000	1.342	1.365	1.354	1.266
2000	0.834	0.900	0.867	0.779
1000	0.557	0.548	0.553	0.465
500	0.325	0.345	0.335	0.247
250	0.253	0.265	0.259	0.171
125	0.146	0.156	0.151	0.063
0	0.087	0.088	0.088