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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-EL004960MO] Mouse CD81 antigen(CD81) ELISA kit

[CSB-EL004960MO] Mouse CD81 antigen(CD81) ELISA kit

제품코드 : CSB-EL004960MO

제품정보 : Mouse CD81 antigen(CD81)

Sample Types: serum, plasma, tissue homogenates, cell lysates
Detect Range: 47 pg/mL-3000 pg/mL
Sensitivity: 11.75 pg/mL

제품문의

전화번호

2292-8870

이메일

dreamcell@dreamcell.co.kr

제품상세

Product Details

Description

This Mouse CD81 ELISA Kit was designed for the quantitative measurement of Mouse CD81 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL.

Target Name

CD81 molecule

Alternative Names

Cd81 ELISA Kit; Tapa1CD81 antigen ELISA Kit; 26 kDa cell surface protein TAPA-1 ELISA Kit; Target of the antiproliferative ELISA Kit 1 ELISA Kit; CD antigen CD81 ELISA Kit

Abbreviation

CD81

Uniprot No.

P35762

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

47 pg/mL-3000 pg/mL

Sensitivity

11.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD81 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-101
1:2	Average %	91
	Range %	85-96
1:4	Average %	84
	Range %	80-88
1:8	Average %	95
	Range %	95-99

Recovery

The recovery of mouse CD81 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	104	100-113

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.692	2.600	2.646	2.497
1500	2.057	1.938	1.998	1.849
750	1.376	1.428	1.402	1.253
375	0.909	0.854	0.882	0.733
187.5	0.589	0.609	0.599	0.450
94	0.365	0.371	0.368	0.219
47	0.239	0.247	0.243	0.094
0	0.152	0.145	0.149