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홈 > 제품정보 > 시약 > 해외 공식 대리점 > [CSB-E12707h] Human Cluster of differentiation 8,CD8 ELISA Kit
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[CSB-E12707h] Human Cluster of differentiation 8,CD8 ELISA Kit

제품코드 : CSB-E12707h

제품정보 : Human Cluster of differentiation 8,CD8

  • Sample Types: serum, plasma, cell culture supernates, tissue homogenates
  • Detect Range: 62.5 U/mL-4000 U/mL
  • Sensitivity: 15.6 U/mL

제품문의

  • 전화번호
    2292-8870

제품상세

Product Details

Description

This Human CD8A ELISA Kit was designed for the quantitative measurement of Human CD8A protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 U/mL-4000 U/mL and the sensitivity is 15.6 U/mL.

Target NameCD8a molecule
Alternative Namesalpha polypeptide (p32) ELISA Kit; CD8 ELISA Kit; CD8 antigen alpha polypeptide ELISA Kit; CD8 antigen alpha polypeptide (p32) ELISA Kit; CD8a ELISA Kit; CD8A antigen ELISA Kit; CD8A molecule ELISA Kit; CD8A_HUMAN ELISA Kit; Leu2 ELISA Kit; Leu2 T lymphocyte antigen ELISA Kit; Ly3 ELISA Kit; LYT3 ELISA Kit; MAL ELISA Kit; OKT8 T cell antigen ELISA Kit; OTTHUMP00000160760 ELISA Kit; OTTHUMP00000160764 ELISA Kit; OTTHUMP00000203528 ELISA Kit; OTTHUMP00000203721 ELISA Kit; p32 ELISA Kit; T cell antigen Leu2 ELISA Kit; T cell co receptor ELISA Kit; T-cell surface glycoprotein CD8 alpha chain ELISA Kit; T-lymphocyte differentiation antigen T8/Leu-2 ELISA Kit; T8 T cell antigen ELISA Kit; T8/Leu-2 T-lymphocyte differentiation antigen ELISA Kit
AbbreviationCD8A
Uniprot No.P01732
SpeciesHomo sapiens (Human)
Sample Typesserum, plasma, cell culture supernates, tissue homogenates
Detection Range62.5 U/mL-4000 U/mL
Sensitivity15.6 U/mL
Assay Time1-5h
Sample Volume50-100ul
Detection Wavelength450 nm
Research AreaImmunology
Assay Principlequantitative
MeasurementSandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%    
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%    
Three samples of known concentration were tested in twenty assays to assess.   
        
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. 
　SampleSerum(n=4)  
1:1Average %94  
Range %86-98  
1:2Average %99  
Range %88-104  
1:4Average %97  
Range %89-100  
1:8Average %105  
Range %94-109  
Recovery
The recovery of human CD8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. 
 
Sample TypeAverage % RecoveryRange  
Serum (n=5)10088-104  
EDTA plasma (n=4)9788-100  
        
        
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
 
U/mlOD1OD2AverageCorrected  
40001.9791.9411.9601.783  
20001.4711.4551.4631.286  
10000.9610.9890.9750.798  
5000.6470.6850.6660.489  
2500.4470.4540.4510.274  
1250.2910.3080.3000.123  
62.50.2270.2320.2300.053  
00.1790.1750.177　  
ELISA Data AnalysisWatch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs		ELISA kit FAQs
StorageStore at 2-8°C. Please refer to protocol.
Lead Time3-5 working days

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